Design and application of a confocal microscope for spectrally resolved anisotropy imaging
نویسندگان
چکیده
منابع مشابه
Design and application of a confocal microscope for spectrally resolved anisotropy imaging.
Biophysical imaging tools exploit several properties of fluorescence to map cellular biochemistry. However, the engineering of a cost-effective and user-friendly detection system for sensing the diverse properties of fluorescence is a difficult challenge. Here, we present a novel architecture for a spectrograph that permits integrated characterization of excitation, emission and fluorescence an...
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A scattering microscope was developed to investigate single cells and biological microstructures by light scattering measurements. The spectrally resolved part of the setup and its validation are shown in detail. The analysis of light scattered by homogenous polystyrene spheres allows the determination of their diameters using Mie theory. The diameters of 150 single polystyrene spheres were det...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2011
ISSN: 1094-4087
DOI: 10.1364/oe.19.002546